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A homogeneous ,fluorescence polarization, assay (FPIA) for ,detection, of bovine antibody to Brucella abortus was validated. 733 serum samples were collected from farms with no evidence of ,brucellosis, and 1039 serum samples from farms where B. abortus was diagnosed in at least one animal using buffered antigen plate agglutination and the competitive enzyme immunoassay.
The invention relates to a brucella ,fluorescence polarization, (FPA) ,detection kit,. In the ,kit,, fluorescein isothiocyanate (FITC) is adopted for marking smooth brucella O-chain lipopolysaccharide micromolecule segment (OPS) and serves as an antigen. On the basis of FPA experimental principle, the ,kit, can be used for detecting ,brucellosis, of various animals and simultaneously detecting IgG and ...
Here we use the ,fluorescence polarization, capabilities of the Synergy™ 2 Multi-Mode Microplate Reader to run Brucella and Mycobacterium bovis antibody ,detection kits, from Diachemix. Introduction Contagious diseases in livestock herds represent a serious public health threat as well as causing significant economic loss.
Fluorescence polarization, immunoassay (FPA) uses molecular rotational properties to measure antibody binding to antigen directly. The potential use of this method was assessed in comparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of ,brucellosis, on a total of 587 human sera.
easy to perform, economical, and is more accurate. The ,Fluorescence Polarization, Test (FP) test, a test that has been used for years in human medicine, has been adapted for use in the field and the laboratory for the ,detection, of ,brucellosis, in cattle, bison, and swine.
1/1/2019, · ,Fluorescence polarization, assay for the diagnosis of swine ,brucellosis, was standardized and evaluated. • Test performances evaluated using representative panel (n = 100) had revealed 95.00% sensitivity and 98.75% specificity. • Evaluation of the test in swine ,brucellosis, outbreak (n = 300) revealed 86.96% sensitivity and 100% specificity.
Protective clothing, and equipment must be decontaminated, cleaned, laundered, maintained, and replaced when no longer useful. Under ... Methods of decontamination ,include isolation, of contaminants, physical removal ... This process entails removing contaminated equipment and ,protective clothing, and leaving it at the decontamination ,area,.
2/4/2014, · Currently, the ,fluorescence polarization, assay (FPA) is a serological method applied for diagnosis of ,brucellosis, both in humans and animals due to it is a quick, homogenous, species-independent technique, which was initially progressed and authenticated for the recognition of antibodies specific to Brucella abortus in cattle.
A simple, rapid, inexpensive ,fluorescence polarization, assay for the ,detection, of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and 95.9%, respectively, is described. The assay detects antibodies to B. abortus in 15 min by testing undiluted whey produced by chemical and physical manipulation of milk from bulk ...
Fluorescence Polarization Detection, of Brucella abortus and Mycobacterium bovis specific antibodies Using ,Kits, from Diachemix Veterinary Diagnosis of ,Brucellosis, and Tuberculosis A p p l i c a t i o n N o t e Paul Held Ph.D., Principle Scientist, BioTek Instruments, Inc., Winooski, VT Michael E. Jolley Ph.D., Diachemix Corporation