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1. Context ,Brucellosis, is a zoonotic disease, endemic in many parts of the world especially the Middle East, South and Central Asia, Mediterranean region, Europe, North and East Africa, and Latin America with over half a million new cases annually (1-3).Clinical management of ,brucellosis, is one of the most challenging obstacles due to a high rate of failure in treatment and subsequent relapse ...
This ,kit, includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either ,fluorescence, resonance energy transfer (FRET) with a standard fluorometer or ,fluorescence polarization, (FP) with capable instrumentation.
Fluorescence polarization, measurements provide information on molecular orientation and mobility and processes that modulate them, including receptor–ligand interactions, protein–DNA interactions, proteolysis, membrane fluidity and muscle contraction (Figure 1).Because ,polarization, is a general property of fluorescent molecules (with certain exceptions such as lanthanide chelates ...
The complement fixation test (CFT), competitive enzyme immunoassay (CELISA), indirect enzyme immunoassay (IELISA) and ,fluorescence polarization, assay (FPA) were evaluated for the ,detection, of antibodies to Brucella abortus and Brucella suis biotype 4 in caribou (Rangifer tarandus caribou), elk (Cervus elapus), red deer (Cervus elapus), and reindeer (Rangifer tarandus tarandus). When combining ...
Catalogue Number 921012 – 1 . ,Fluorescence polarization, assay for ,detection, of antibody to smooth Brucella sp. in most animal hosts and man. This assay is based on the technique described in the OIE Manual of Diagnostic Tests and Vaccines, 2008 edition as a prescribed test for international trade.
Brucellosis, in livestock causes enormous losses for economies of developing countries and poses a severe health risk to consumers of dairy products. Little information is known especially on camel ,brucellosis, and its impact on human health. For surveillance and control of the disease, sensitive and reliable ,detection, methods are needed. Although serological tests are the mainstay of diagnosis ...
Application of serum based PCR and ,fluorescence polarization, assay for diagnosis of ,brucellosis, among people occupationally at risk to disease. Int. J. Agric. Biol., 18: 377‒384 Application of Serum Based PCR and ,Fluorescence Polarization, Assay for Diagnosis of ,Brucellosis, among People Occupationally at …
NIELSEN, K. et al. Diagnosis of bovine ,brucellosis, using a homogeneous ,fluorescence polarization, assay. Veterinary Immunology and Immunopathology, v.66, p.321-329, 1998. [ Links ] NIELSEN, K. et al. ,Fluorescence polarization, assay for the diagnosis of bovine ,brucellosis,: adaptation to field use.
2/4/2014, · Currently, the ,fluorescence polarization, assay (FPA) is a serological method applied for diagnosis of ,brucellosis, both in humans and animals due to it is a quick, homogenous, species-independent technique, which was initially progressed and authenticated for the recognition of antibodies specific to Brucella abortus in cattle.
evaluation of the ,fluorescence polarization, assay and comparison to other serological assays for ,detection, of ,brucellosis, in cervids D. Gall 1, 5 , K. Nielsen 1 , L. Forbes 2 , W. Cook 3 , D. Leclair 4 , S. Balsevicius 1 , L. Kelly 1 , P. Smith 1 , and M. Mallory 1